Leon Bodzin

Leon Bodzin

San Diego, California, United States
127 followers 122 connections

About

An astute Senior Software Engineer having extensive experience building high-performance…

Activity

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Experience

  • Northrop Grumman Graphic

    Northrop Grumman

    Rancho Bernardo, California

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    San Diego, California

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    Carlsbad, California

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    San Diego, California

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    San Diego, California

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    San Diego, California

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    La Jolla, California

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    San Diego, California

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    San Diego, California

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    San Diego, California

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    San Diego, California

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    Panama City, Florida

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Education

  • Florida Atlantic University Graphic

    Florida Atlantic University

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    Activities and Societies: Society of Naval Architects & Marine Engineers (SNAME)

    First Place winner of engineering week competition for presentation of "Deep Ocean Dumping in the New York Bight with Emphasis on the Acoustic Monitoring Technique". Recipient of International Yachtsmen Association Scholarship. Attained Engineer-in-Training (EIT) certification.

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Publications

  • Automated In Situ Measurement of Cell-Specific Antibody Secretion and Laser-Mediated Purification for Rapid Cloning of Highly-Secreting Producers

    Biotechnology and Bioengineering

    Cloning of highly-secreting recombinant cells is critical for biopharmaceutical manufacturing, but faces numerous challenges including the fact that secreted protein does not remain associated with the producing cell. A fundamentally new approach was developed combining in situ capture and measurement of individual cell protein secretion followed by laser-mediated elimination of all non- and poorly-secreting cells, leaving only the highest-secreting cell in a well. Recombinant cells producing…

    Cloning of highly-secreting recombinant cells is critical for biopharmaceutical manufacturing, but faces numerous challenges including the fact that secreted protein does not remain associated with the producing cell. A fundamentally new approach was developed combining in situ capture and measurement of individual cell protein secretion followed by laser-mediated elimination of all non- and poorly-secreting cells, leaving only the highest-secreting cell in a well. Recombinant cells producing humanized antibody were cultured serum-free on a capture matrix, followed by staining with fluorescently-labeled anti-human antibody fragment. A novel, automated, high-throughput instrument (called LEAP) was used to image and locate every cell, quantify the cell-associated and secreted antibody (surrounding each cell), eliminate all undesired cells from a well via targeted laser irradiation, and then track clone outgrowth and stability. Temporarily sparing an island of helper cells around the clone of interest improved cloning efficiency (particularly when using serum-free medium), and helper cells were easily eliminated with the laser after several days. The in situ nature of this process allowed several serial sub-cloning steps to be performed within days of one another, resulting in rapid generation of clonal populations with significantly increased and more stable, homogeneous antibody secretion. Cell lines with specific antibody secretion rates of > 50 pg/cell per day (in static batch culture) were routinely obtained as a result of this cloning approach, often times representing up to 20% of the clones screened.

    6/28/2006: ACKNOWLEDGED BY: Szaniszlo P., Rose W.A., Wang N., Reece L.M., Tsulaia T.V., Hanania E.G., Elferink C.J., Leary J.F. Scanning Cytometry with a LEAP: Laser-Enabled Analysis and Processing of Live Cells In Situ. Cytometry A. 2006;69:641–651. See: http://banix.com/docs/2006-InternationalSocietyForAnalyticalCytology.pdf

    Other authors
    • Elie G. Hanania
    • Annabeth Fieck
    • Janine Stevens
    • Bernhard O. Palsson
    • Manfred R. Koller
    See publication
  • TOGA: An Automated Parsing Technology for Analyzing Expression of Nearly All Genes

    Proceedings of the National Academy of Sciences

    We have developed an automated, high-throughput, systematic cDNA display method called TOGA, an acronym for total gene expression analysis. TOGA utilizes 8-nt sequences, comprised of a 4-nt restriction endonuclease cleavage site and adjacent 4-nt parsing sequences, and their distances from the 3' ends of mRNA molecules to give each mRNA species in an organism a single identity. The parsing sequences are used as parts of primer-binding sites in 256 PCR-based assays performed robotically on…

    We have developed an automated, high-throughput, systematic cDNA display method called TOGA, an acronym for total gene expression analysis. TOGA utilizes 8-nt sequences, comprised of a 4-nt restriction endonuclease cleavage site and adjacent 4-nt parsing sequences, and their distances from the 3' ends of mRNA molecules to give each mRNA species in an organism a single identity. The parsing sequences are used as parts of primer-binding sites in 256 PCR-based assays performed robotically on tissue extracts to determine simultaneously the presence and relative concentration of nearly every mRNA in the extracts, regardless of whether the mRNA has been discovered previously. Visualization of the electrophoretically separated fluorescent assay products from different extracts displayed via a Netscape browser-based graphical user interface allows the status of each mRNA to be compared among samples and its identity to be matched with sequences of known mRNAs compiled in databases.

    Other authors
    • J. Gregor Sutcliffe
    • Pamela E. Foye
    • Mark G. Erlander
    • Brian S. Hilbush
    • Jayson T. Durham
    • Karl W. Hasel
    See publication

Patents

  • Internal Conduit Vehicle

    Issued US 5,551,349

    An internal conduit vehicle comprises two coaxially aligned dual-vector wheels mounted to each end of a chassis. The wheels include multiple, elongated rollers each positioned at an angle around a hub assembly. Two motors mounted to opposite ends of the chassis are rotationally coupled to a hub assembly. A motor control system mounted to the chassis drives the motors so that the wheels counter-rotate with equal, but opposite torque, thereby driving the vehicle axially through a conduit.

    See patent
  • Child's Banister Guard

    Issued US 5,076,545

    A child's safety guard for baluster-type banisters designed to prevent any portion of a child's body from falling through or being jammed between adjacent balusters and railings, without degrading the aesthetic appearance of the banisters. The guard comprises a set of thin, substantially stiff panels of sheet material having a parallelogram configuration capable of being adjustably overlapped along adjacent edges to cover substantially the entire space facing the landing between the railings…

    A child's safety guard for baluster-type banisters designed to prevent any portion of a child's body from falling through or being jammed between adjacent balusters and railings, without degrading the aesthetic appearance of the banisters. The guard comprises a set of thin, substantially stiff panels of sheet material having a parallelogram configuration capable of being adjustably overlapped along adjacent edges to cover substantially the entire space facing the landing between the railings. The panels are secured in their overlapped position by a lacing threaded through a series of drilled openings along the top and bottom edges of the panels and around the balusters. The panels are preferably fabricated of a clear, plastic material, but can be made in any desired color.

    Other inventors
    • Alice Bodzin (Honorary)
    See patent
  • Adjustable Frequency Diversity Acoustic Communications System

    Issued US 5,018,114

    An underwater acoustic communication system provides an operator with adjable frequency diversity so that data rates may be tailored to specific multipath environments. Transmitted messages are sent with precursor transmission/reception synchronization data and transmission parameter data so that the receiving communication end will recognize when message data starts, tone length as-well-as the frequency diversity of the transmitted message. Timing is extracted from the data to compensate for…

    An underwater acoustic communication system provides an operator with adjable frequency diversity so that data rates may be tailored to specific multipath environments. Transmitted messages are sent with precursor transmission/reception synchronization data and transmission parameter data so that the receiving communication end will recognize when message data starts, tone length as-well-as the frequency diversity of the transmitted message. Timing is extracted from the data to compensate for Doppler shift.

    Other inventors
    • Gerald R. Mackelburg
    • Howard B. McCracken
    • Anthony E. Diamond
    See patent
  • Apparatus for Reading Signals Generated from Resonance Light Scattered Particle Labels

    Filed US 12/356,975

    Embodiments of the present invention include a control and analysis system, a signal generation and detection apparatus, or reader for capturing, processing and analyzing images of samples having resonance light scattering (RLS) particle labels. An analyzer/reader includes an illumination system having a unique shutter/aperture assembly for delivering precise patterns of light to a sample, a computer controlled X-Y stage, and a detection system comprising a CCD camera to allow separation and…

    Embodiments of the present invention include a control and analysis system, a signal generation and detection apparatus, or reader for capturing, processing and analyzing images of samples having resonance light scattering (RLS) particle labels. An analyzer/reader includes an illumination system having a unique shutter/aperture assembly for delivering precise patterns of light to a sample, a computer controlled X-Y stage, and a detection system comprising a CCD camera to allow separation and analysis of detected light that contains information from gold and/or silver RLS labels. Alternative embodiments include linear scanning apparatus and simplified apparatus for low density samples.

    Other inventors
    • Juan Yguerabide
    • Laurence Warden
    • Todd Peterson
    • Dirk Tenbroeck
    See patent
  • Method and Apparatus for Normalization and Deconvolution of Assay Data

    Filed US 11/835,982

    The present invention is directed to deconvolution and normalization of assay data. The present invention includes a control and analysis system, used in conjunction with a signal generation and detection apparatus, for capturing, processing and analyzing images of samples having resonance light scattering (RLS) particle labels. The control and analysis system processes instructions and algorithms for performing multiplexed assays of two or more colors, for example, to allow separation and…

    The present invention is directed to deconvolution and normalization of assay data. The present invention includes a control and analysis system, used in conjunction with a signal generation and detection apparatus, for capturing, processing and analyzing images of samples having resonance light scattering (RLS) particle labels. The control and analysis system processes instructions and algorithms for performing multiplexed assays of two or more colors, for example, to allow separation and analysis of detected light that contains information from two or more different types or sizes of RLS particles. The multiplexing analysis software is preferably incorporated within the system of the present invention, and the multiplexing analysis is preferably performed in real-time during a scanning or assay procedure. The invention provides for a computer readable medium containing instructions for carrying out the same.

    Other inventors
    • Juan Yguerabide
    • Laurence Warden
    • Richard R. Anderson
    • Kate Rhodes
    See patent
  • Method and System for Multiple Deployments of Electronic Information

    Filed US US0116258

    A method and system for multiple deployment of electronic information in an electronic data warehouse. New deployments of electronic information are built into a file system for an electronic data warehouse on a network device on a computer network. A deployment includes electronic information as well as other components (e.g., tools, action scripts, etc.) that are used to process, manipulate and/or provide access to the electronic information. Access to a deployment is provided with an access…

    A method and system for multiple deployment of electronic information in an electronic data warehouse. New deployments of electronic information are built into a file system for an electronic data warehouse on a network device on a computer network. A deployment includes electronic information as well as other components (e.g., tools, action scripts, etc.) that are used to process, manipulate and/or provide access to the electronic information. Access to a deployment is provided with an access view. The access view includes a list of components from the deployment that can be accessed via the access view. The access view is used to switch from a first access point in a hierarchical structure for a first deployment to a second access point in a hierarchical structure for a second deployment. Switching between deployments allows electronic information to be compared between deployments. The methods and system described herein may be used as new bioinformatic techniques to manipulate and store multiple deployments of biotechnology data used for simultaneous sequence-specific identification of expressed genes.

    See patent

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